Wilfred Chen
Presidential Chair
Professor of Chemical and Environmental Engineering
Faculty Member, Environmental Toxicology Graduate Program
Faculty Member, Microbiology Program
RESEARCH AND EDUCATION | RESEARCH TOPICS | RESEARCH FUNDING | PUBLICATIONS | HONORS |
GROUP MEMBERS |
RESEARCH AND EDUCATION
·
B.S. in Chemical Engineering, University of California Los Angeles
·
Ph.D. in Chemical Engineering, California Institute of Technology, 1993
·
Postdoctoral Research Associate, Institute of Biotechnology
Institute of Technology , Zurich, Switzerland, 1993-1994
· NSF Career Award, 1997
· Editorial Board: Biotechnology and Bioengineering, 2/03-Present; Applied and Environmental Microbiology, 1/04-Present; Microbial Cell Factories, 6/04-Present; Journal of Biological Engineering, 1/07-Present
· Associate Editor: Applied Biochemistry and Biotechnology, July 03-Present; Biochemical Engineering Journal, 3/08-Present
RESEARCH TOPICS
Over the past three decades breakthroughs in molecular genetics have revolutionized our ability to analyze and manipulate the structures and properties of nucleic acids and proteins. The combination of chemical engineering principles and molecular biology will be the foundation of an increasing number of problems, ranging from environmental monitoring and cleanup to the production of high value products. My current research efforts are focused on the development of novel biocatalysts and biological materials that can be used to enhance or manipulate microbial metabolism for the degradation of toxic pollutants or for environmentally-friendly chemical synthesis. Another area of interest is the application of these novel biocatalysts or biomaterials for biosensor development.
Environmental Biotechnology
One area of interest is the detoxification of organophosphate nerve agents. Organophosphorus hydrolase (OPH), an enzyme that hydrolyzes organophosphates efficiently, was used to develop several microbial- and enzyme-based detoxification strategies. These strategy are suitable for development of low cost detoxification processes for the degradation of stored or spilled organophosphate wastes.
Another area of interest is the remediation of heavy metal contamination. Both microbial- and biopolymer-based technologies were developed. Synthetic phytochelatins (Glu-Cys)nGly (ECs) are cysteine-rich peptides that bind metal ions and sequester them in biological inactive forms. We demonstrated that bacterial cells with ECs anchored on the cell surface accumulated up to 30-fold more cadmium and mercury than wild-type cells. We are in the process of developing a novel rhizoremediation approach for the removal of TCE and heavy metals from contaminated soil.
Arsenic contamination is a major drinking water problem that adversely affects the health of many people over the world. A new remediation strategy is currently being investigated by developing a metabolically engineered yeast strain for enhanced uptake and accumulation of arsenic. We believe this strategy will provide a low-cost and efficient technology for arsenic removal.
Nanoscale materials have been gaining increasing interest in the area of environmental remediation because of their unique physical, chemical and biological properties. With the advent of recombinant DNA techniques, it is now possible to create “artificial” protein polymers with fundamentally new molecular organization that can be controlled precisely in terms of sizes, compositions and functions at the molecular level. In this research, nanoscale biopolymers with both metal-binding and tunable properties were designed for the selectively removal of heavy metals from dilute waste streams. By tuning the process temperature, reversible network formation between the individual biopolymer enables the recovery of sequestered heavy metals such as cadmium, mercury and arsenic by precipitation.
Biosensor
Biosensors are analytical tools or systems consisting of an immobilized biological sensing material in close contact with a suitable transducer that converts the biochemical signal to a quantifiable electrical signal. Presently on going projects are: (1) development of enzyme and microbial biosensors for selective, rapid and direct monitoring of organophosphate pesticides and nerve agents; (2) development of novel molecular beacon-based DNA-probes for monitoring pathogens and viruses in drinking water, (3) Sensor arrays for the rapid and sensitive detection of a complex mixture of chemical and biological agents. .
RESEARCH FUNDING
Environmental
Biotechnology
A. . Heavy Metal Removal
· NSF, 9/1/03-8/31/08
· Metropolitan Water District of Southern California, 3/15/04-9/30/08
· NSF, 9/1/04-8/31/08
C. Monitoring of Environmental Pathogens and
Microbes
· EPA, 9/1/06-8/31/09
· UC Water Resources, 7/1/08-6/30/10
Drug Screening
A. Genetically Engineered Modules for Protease inhibitors screening
· NSF, 4/15/08-3/1/11
Sensor
A. Antibody Sensor Array
· NSF, 9/1/06-8/31/09
B. Nanowire Sensor Array
· UCR Center for Nanoscale Science and Engineering, 1/15/07-8/31/08
· US EPA , 7/1/05-6/30/09
· NSF , 9/1/05-8/31/08
· UC-LLNL , 9/1/08-8/31/09
PUBLICATIONS
163. C.L. Aravinda, Serge Cosnier, Wilfred Chen, Nosang V. Myuing, and Ashok Mulchandani, Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemi-resistive sensor, Biosensors and Bioelectronics, Accepted for Publication.
162. Shailendra Singh, Seung Hyun Kang, Ashok Mulchandani and Wilfred Chen, Bioremediation: Environmental clean up through pathway engineering, Curr. Opin. Biotechnol., Accepted for Publication. (Invited Contribution)
161. Chao Yang, Qiao Zhao, Zheng Liu, Qiyun Li, Chuanling Qiao, Ashok Mulchandani, and Wilfred Chen, Cell surface display of functional macromolecule fusions on Escherichia coli for development of a bioluminescent whole-cell biocatalyst, Environ. Sci. Technol., 42, 6105-6110, 2008. pdf
160. Maxwell C. Kum, Bong Young Yoo, Young Woo Rheem, Krassimir N. Bozhilov, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, Synthesis and characterization of cadmium telluride nanowire, Nanotechnology, 19, 325711, 2008. pdf
159. Seung Hyun Kang, Krassimir N. Bozhilov, Nosang V. Myung, Ashok Mulchandani and Wilfred Chen, Microbial synthesis of CdS nanocrystals in genetically engineered E. coli, Angewandte Chemie International Edition, 47, 5186 –5189, 2008. pdf
158. Adam K. Wanekaya, Wilfred Chen, Ashok Mulchandani, Recent biosensing developments in environmental security, J. Environ. Monitoring, 10, 703–712, 2008. pdf
157. S. Sandhya, Wilfred Chen and Ashok Mulchandani, Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Escherichia coli from Fresh Produce and Water, Analytica Chimica Acta, 614, 208-212, 2008. pdf
156. Lianbin Xu, Zhongwei Chen, Wilfred Chen, Ashok Mulchandani, and Yushan Yan, Electrochmical synthesis of perfluorinated ions doped coducting polyaniline films consisting of helical fibers and their reversible switching between superhydrophobicity and superhydrophilicity, Macromolecular Rapid Communications, 29, 832–838, 2008. pdf
155. Cindy H. Wu, Yu-Chen Hwang, Wonkyu Lee, Ashok Mulchandani, Thomas K. Wood, Marylynn V. Yates, and Wilfred Chen, Detection of recombinant Pseudomonas putida biofilm in wheat rhizosphere by two-colored fluorescence in situ hybridization, Appl. Microbiol. Biotechnol., 79, 511–518, 2008. pdf
154. Shailendra Singh, Ashok Mulchandani, and Wilfred Chen, Highly Selective and Rapid Arsenic Removal by Metabolically Engineered E. coli Expressing Fucus vesiculosus Metallothionein, Appl. Environ. Microbiol., 74, 2924–2927, 2008. pdf
153. Hsiao-Yun Yeh, Yu-Chen Hwang, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Detection of Hepatitis A Virus By Using a Combined Cell-Culture – Molecular Beacon Assay, Appl. Environ. Microbiol., 74, 2239-2243, 2008. pdf
152. Cindy H. Wu, Ashok Mulchandani, and Wilfred Chen, Versatile microbial surface-display for environmental remediation and biofuels production, Trends in Microbiology, 16, 181-188, 2008. pdf
151. Carlos N. Hangarter, Mangesh Banger, Sandra C. Hernandez, Wilfred Chen, Marc A. Deshusses, Ashok Mulchandani, and Nosang V. Myung, Maskless electrodeposited contact for conducting polymer nanowires, Appl. Phys. Letts, 92, 073104. 2008. pdf
150. Yu-Chen Hwang, Justin Jang-Hann Chu, Priscilla L. Yang, Wilfred Chen, and Marylynn V. Yate, A Cellular Sensor for Screening Small Molecules Suppressing Poliovirus Replication, Antiviral Research, 77, 232–236, 2008. pdf
149. U Loi Lao, Minwei Sun, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen, Genetic engineering of self-assembled protein hydrogel based on elastin-like sequences with metal binding functionality, Biomacromolecules, 8, 3736-3739, 2007. pdf
148. Maxwell Kum, Kanchan A. Joshi, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Biomolecules-carbon nanotubes doped conducting polymer composites and their sensor application, Talanta, 74, 370-375, 2007. pdf
147. Joun S. Lee, Aijun A. Wang, Bongyoung Yoo, Youngwoo Rheem, Ashok Mulchandani, Wilfred Chen and Nosang V. Myung, DNA Assisted Assembly of Multi-Segmented Nanowires, Electroanalysis, 22, 2287-2293, 2007. pdf
146. Seung Hyun Kang, Shailendra Singh, Jae-Young Kim, Wonkyu Lee, Ashok Mulchandani, and Wilfred Chen, Bacteria Metabolically Engineered for Enhanced Phytochelatin Production and Cadmium Accumulation, Appl. Environ. Microbiol., 73, 6317-6320, 2007. pdf
145. Shailendra Singh, Wonkyu Lee, Nancy A. DaSilva, Ashok Mulchandani, and Wilfred Chen, Enhanced arsenic accumulation and removal by engineered yeast cells expressing Arabidopsis thaliana phytochelatin synthase, Biotechnol. Bioeng., 99, 333-340, 2008. pdf
144. Can Attila, Akihiro Ueda, Suat L.G. Cirillo, Jeffery D. Cirillo, Wilfred Chen, and Thomas K. Wood, Pseudomonas aeruginosa PAO1 virulence factors and poplar tree response in the rhizosphere, Microbial Biotechnology, 1, 17-29, 2008. pdf
143. Sandra C. Hernandez, Debangshu Chaudhuri, Wilfred Chen, Nosang Myung, and Ashok Mulchandani, Single polypyrrole nanowire ammonia gas sensor, Electroanalysis, 19, 2125-2130, 2007. pdf
142. Chaokun Li, Yaran Zhu, Inga Benz, M. Alexander Schmidt, Wilfred Chen, Ashok Mulchandani, and Chuanling Qiao, Presentation of Functional Organophosphorus Hydrolase Fusions on the Surface of Escherichia coli by the AIDA-I Autotransporter Pathway, Biotechnol. Bioeng., 99, 485-490, 2008. pdf
141. Chao Yang, Ning Cai, Ming Dong, Hong Jiang, Jinming Li Chuanling Qiao, Ashok Mulchandani, Wilfred Chen, Surface Display of MPH on Pseudomonas putida JS444 Using Ice Nucleation Protein and Its Application in Detoxification of Organophosphate, Biotechnol. Bioeng., 99, 30-37, 2008. pdf
140. U Loi Lao, Alin Chen, Mark R. Matsumoto, Ashok Mulchandani, Wilfred Chen, Cadmium Removal from Contaminated Soil by Thermally Responsive Elastin (ELPEC20) Biopolymer, Biotechnol. Bioeng., 98, 349-355, 2007. pdf
139. U Loi Lao, Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Affinity Purification of Plasmid DNA by Temperature-Triggered Precipitation, Nature Protocols, 2, 1263-1268, 2007. (Invited Contribution) pdf
138. Y-Z Zheng, W-S. Lan, C-L. Aiao, A. Mulchandani, and W. Chen, Decontamination of vegetables sprayed with organophosphate pesticides by organophosphours hydrolase and carboxylesterase (B1), Appl. Biochem. Biotechnol., 136, 233-242, 2007. pdf
137. Yu Lei, Priti Mulchandani, Wilfred Chen, and Ashok Mulchandani, Biosensor for direct determination of Fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface-expressed organophosphorous hydrolase, Appl. Biochem. Biotechnol., 136, 243-250, 2007. pdf
136. Yu-Chen Hwang, Oymon M. Leong, Wilfred Chen, and Marylynn V. Yates, Comparison of a Reporter Assay and Immunomagnetic Separation Real-Time RT-PCR for the Detection of Enteroviruses in Seeded Environmental Water Sample, Appl. Environ. Microbiol., 73, 2338-2340, 2007. pdf
135. Adam Wanekaya, Mangesh A. Banger, Minhee Yun, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Field-Effect Transitors Based on Single Nanowires of Conducting Polymers, J. Phys. Chem. C, 111, 5218-5221, 2007. pdf
134. Gabriela Jenikova, U Loi Lao, Di Gao, Ashok Muchaldani and Wilfred Chen, Elastin-Calmodulin Scaffold for Protein Microarray Fabrication, Langmuir, 23, 2277-2279, 2007. pdf
133. Di Gao, Wilfred Chen, Ashok Mulchandani, and Jerome S. Schultz, Detection of Tumor Markers Based on Extinction Spectra of Visible Light Passing through Gold Nanoholes, Appl. Phys. Letts, 90, 073901, 2007. pdf
132. Kumaran Ramanathan, Mangesh A. Bangar, Minhee Yun, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, In Situ Fabricaton of Single Poly(methyl pyrrole) Nanowire, Electroanalysis, 19, 793-797, 2007. pdf
131. Xianpeng Cai, Ningyi Liu, Yu Lei, Qing Zhang, Mary B. Chan, Changming Li, Wilfred Chen, and Ashok Mulchandani, Single-walled carbon nanotube based real-time organophosphate detector, Electroanalysis, 19, 616-619, 2007. pdf
130. Chandana Karnati, Hongwei Du, Hai-Feng Ji, Yuri Lvov, Wilfred Chen, Ashok Mulchandani, Organophosphrus Hydrolyase Multilayer Modified Microcantilevers for Organophosphorus Detection, Biosensor & Bioelectronics, 22, 2636-2642, 2007. pdf
129. W.S. Lan, J.D. Gu, J.L. Zhang, B.C. Shen, H. Jiang, A. Mulchandani, W. Chen, C.L. Qiao, Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium, International Biodeterioration & Biodegradation, 58, 70–76, 2006. pdf
128. U Loi Lao, Ashok Mulchandani, and Wilfred Chen, Simple Conjugation and Purification of Quantum Dot-Antibody Complexes Using A Thermally Responsive Elastin-Protein L Scaffold As Immunofluorescent Agents, J. Am. Chem. Soc., 128, 14756-14757, 2006. pdf
127. Aijun A. Wang, Joun Lee, Gabriela Jenikova, Ashok Mulchandani, Nosang V. Myung, and Wilfred Chen, Controlled Assembly of Multi-Segment Nanowires by Histidine-Tagged Peptides, Nanotechnology, 17, 3375-3379, 2006. pdf
126. Yu Lei, Priti Mulchanani, Wilfred Chen, and Ashok Mulchandani, Biosensor for direct determination of Fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface expressed organophosphorus hydrolase. 1. Modified clark oxygen electrode, Sensors, 6, 466-472, 2006. pdf
125. Jintae Lee, Li Cao, Saw Yen Ow, Martin E. Barrios-Lierena, Wilfred Chen, Thomas K. Wood, and Philip C. Wright, Proteome Changes after Metabolic Engineering to Enhance Aerobic Mineralization of cis-1,2-Dichloroethylene, J. Proteome Research, 5, 1388-1397, 2006. pdf
124. Katsumi Takayama, Shin-ichiro Suye, Kouichi Kuroda, Mitsuyoshi Ueda, Tetsuya Kitaguchi, Kouta Tsuchiyama, Takeshi Fukuda, Wilfred Chen, and Ashok Mulchandani, Surface Display of Organophosphorus Hydrolase on Saccharomyces cerevisiae, Biotechnol. Prog., 22, 939-943, 2006. pdf
123. Adam Wanekeya, Yu Lei, Elena Bekyarova, Wilfred Chen, Robert Haddon, Ashok Mulchandani, and Nosang V. Myung, Fabrication and properties of conducting polypyrrole/SWNT-PABS composite films and nanotubes, Electroanalysis, 11, 1047-1054, 2006. pdf
122. Wonkyu Lee, Thomas K. Wood, and Wilfred Chen, Engineering TCE-Degrading Rhizobacteria for Heavy Metal Accumulation and Enhanced TCE Degradation, Biotechnol. Bioeng., 95, 399-403, 2006. pdf
121. Yu-Chen Hwang, Wilfred Chen, and Marylynn V. Yates, Using Fluorescence Resonance Energy Transfer for Rapid Detection of Enteroviral Infection In Vivo, Appl. Environ. Microbiol., 72, 3710-3715, 2006. pdf
120. Li Cao, Jintae Lee, Wilfred Chen, and Thomas K. Wood, Enantioconvergent Production of (R)-1-Phenylethane-1,2-Diol from Styrene Oxide by Combining the Solanum tuberosum and an Evolved Agrobacterium radiobacter AD1 Epoxide Hydrolase, Biotechnol. Bioeng., 94, 522-529, 2006. pdf
119. Ayman H. Mansee, Wilfred Chen, and Ashok Mulchandani, Detoxification of the organophosphate nerve agent coumaphos using organophosphorus hydrolase immobilized on cellulose materials, J. Ind. Microbiol. Biotechnol., 32, 554-560, 2005. pdf
118. Adam K. Wanekaya, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Nanowire-Based Electrochemical Biosensor, Electroanalysis, 18, 533-550, 2006. pdf
117. Di Gao, Nicole McBean, Jerome S. Schultz, Yushan Yan, Ashok Mulchandani, Wilfred Chen, Fabrication of Antibody Arrays Using Thermally Responsive Elastin Fusion Proteins, J. Am. Chem. Soc., 128, 676-677, 2006. pdf
116. Catherine Mee-Hie Cho, Ashok Mulchandani, and Wilfred Chen, Functional Analysis of organophosphorus hydrolase variants with high degradation activity toward organophosphate pesticides, Protein Engineering, Design, and Selection, 19, 99-105, 2006. pdf
115. Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Microbial Biosensor for Direct Determination of Nitrophenyl Substituted Organophosphates Using Recombinant Moraxella sp., Analytica Chimica Acta, 568, 217-221, 2006. pdf
114. Yu Lei, Wilfred Chen and Ashok Mulchandani, Microbial Biosensors: A Review, Analytica Chimica Act, 568, 200-210, 2006. pdf
113. Cindy H. Wu, Thomas K. Wood, Ashok Mulchandani, and Wilfred Chen, Engineering plant-microbe symbiosis for rhizoremediation of heavy metal, Appl. Environ. Microbiol., 72, 1129-1134, 2006. pdf
112. Alberto Sanchez Arribas, Terannie Vazquez, Joseph Wang, Ashok Mulchandani, and Wilfred Chen, Electrochemical and optical bioassays of nerve agents based on the organophosphorus-hydrolase mediated growth of cupric ferrocyanide nanoparticles, Electrochemistry Communications, 7, 1371–1374, 2005. pdf
111. Kanchan A. Joshi, Marek Prouza, Maxwell Kum, Joseph Wang, Robert Haddon, Wilfred Chen, and Ashok Mulchandani, Direct detection of V-type nerve agents using a carbon nanotubes and OPH modified amperometric enzyme electrode, Anal. Chem., 78, 331-336, 2006. pdf
110. Zihui Meng, Wilfred Chen, and Ashok Mulchandani, Removal of estrogenic pollutants from contaminated waters using molecularly imprinted polymers, Environ. Sci. Technol., 39, 8958-8962, 2005. pdf
109. Aijun Wang, Andre M. Salazar, Marylynn V. Yates, Ashok Mulchandani, and Wilfred Chen, Visualization and detection of infectious coxsackievirus replication using a combined cell culture-molecular beacon assay, Appl. Environ. Microbiol., 71, 8397-8401, 2005. pdf
108. Yu Lei, Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Highly sensitive and selective amperometric microbial biosensor for direct determination of p-nitrophenyl substituted organophosphate nerve agents, Environ. Sci. Technol., 39, 8853-8857, 2005. pdf
107. Cory O’Neil, Derek Beving, Wilfred Chen, and Yushan Yan, Durability of hydrophilic and antimicrobial zeolite coatings for gravity-independent water separation, AIHCE J., 52, 1157-1161, 2006. pdf
106. Khaled H. Abd El Galil, M. A. El Sokkary, S. M. Kheira, Andre M. Salazar, Marylynn V. Yates, Wilfred Chen and Ashok Mulchandani, Real-Time Nucleic Acid Sequence-Based Amplification (NASBA) assay for the Detection of Hepatitis A virus, Appl. Environ. Microbiol., 71, 7113-7116, 2005. pdf
105. Lianbin Xu, Wilfred Chen, Ashok Mulchandani, Yushan Yan, Reversible Superhydrophobic to Superhydrophilic Conversion of Conducting Polymer Film, Angewandte Chemie International Edition, 44, 6009 –6012, 2005. pdf
104. Jan Kostal, Giridhar Prabhukumar, U Loi Lao, Alin Chen, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen, Customizable Biopolymers for Heavy Metal Remediation, J. Nanoparticle Research, 7, 517-523, 2005. pdf (Invited contribution)
103. Gunjan Goel, Ashwani Kumar, A.K. Puniya, Wilfred Chen, and Kishan Singh, Molecular beacons: A multitask probe, J. Appl. Microbiol., 99, 435-442, 2005. pdf
102. Yu Lei, Ashok Mulchandani, and Wilfred Chen, Improved Degradation of Organophosphorus Nerve Agents and p-Nitrophenol by Pseudomonas putida JS444 with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Prog., 21, 678-681, 2005. pdf
101. Lingyun Rui, Li Cao, Wilfred Chen, Kenneth F. Reardon, and Thomas K. Wood, Protein Engineering of Epoxide Hydrolase from Agrobacterium radiobacter AD1 for Enhanced Activity and Enantioselective Production of (R)-1-Phenylethane-1,2-Diol, Appl. Environ. Microbiol., 71, 3995-4003, 2005. pdf
100. Aijun A. Wang, Wilfred Chen, and Ashok Mulchandani, Detoxification of organophosphate nerve agents by immobilized dual functional biocatalysts in a cellulose hollow fiber bioreactor, Biotechnol. Bioeng., 91, 379-386, 2005. pdf
99. Wilfred Chen, Ashok Mulchandani, and Marc Deshusses, Environmental Biotechnology: Challenges and Opportunities for Chemical Engineers, AICHE J., 51, 690-695, 2005. pdf
98. Wilfred Chen and Ashok Mulchandani. Engineering of Improved Biocatalysts in Bioremediation. Soil Biology, Volume 2, Biodegradation and Bioremeidation. A. Singh and O.P. Ward (Ed.). Springer-Verlag Berlin Heidelberg. Pp. 235-250, 2004.
97. Jae-Young Kim, Sean O’Malley, Ashok Mulchandani, and Wilfred Chen, Genetically engineered elastin-protein A fusion as a universal platform for homogeneous, phase-separation immunoassay, Anal. Chem., 77, 2318-2322, 2005. pdf
96. Jae-Young Kim, Ashok Mulchandani, and Wilfred Chen, Temperature-triggered purification of antibodies, Biotechnol. Bioeng., 90, 373-379, 2005. pdf
95. Kumaran Ramanathan, Mangesh A. Bangar, Minhee Yun, Wilfred Chen, Nosang V. Myung, and Ashok Mulchandani, Bioaffinity Sensing Using Biologically-Functionalized Conducting Polymer Nanowire, J. Am. Chem. Soc., 127, 496-497, 2005. pdf
94. Priti Mulchandani, Carlos Hangtarer, Yu Lei, Wilfred Chen, and Ashok Mulchandani, Amperometric microbial biosensor for p-nitrophenol using Moraxella sp.-modified carbon paste electrode, Biosensors and Bioelectronics, 21, 523-527, 2005. pdf
93. Yu Lei, Priti Mulchandani, Wilfred Chen and Ashok Mulchandani, Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using recombinant pseudomonas putida JS444-modified clark oxygen electrode, J. Agri. Food Chem., 53, 524-527, 2005. pdf
92. Randhir Prakash Deo, Joseph Wang, Ines Block, Ashok Mulchandani, Kanchan A. Joshi, Marek Trojanowicz, Fritz Scholz, Wilfred Chen, and Yuehe Lin, Determination of organophosphate pesticides at a carbon nanotube/organophosphorus hydrolase electrochemical biosensor, Analytica Chimica Acta, 530, 185-189, 2005. pdf
91. Lingyun Rui, Li Cao, Wilfred Chen, Kenneth F. Reardon, and Thomas K. Wood, Active Site Engineering of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 to Enhance Aerobic Mineralization of cis-1,2-Dichloroethylene in Cells Expressing an Evolved Toluene ortho Monooxygenase, J. Biol. Chem., 279, 46810-46817, 2004. pdf
90. Erica S. Forzani, Haiqian Q. Zhang, Wilfred Chen and Nongjian Tao, Detection of heavy metal ions in drinking water using a high-resolution differential Surface Plasmon Resonance Sensor, Environ. Sci. Technol., 39, 1257-1262, 2005. pdf
89. Andrew M. P. McDonnell, Derek Beving, Albert Wang, Wilfred Chen, and Yushan Yan, Hydrophilic and antimicrobial zeolite coatings for gravity-independent water separation, Adv. Funct. Mat., 15, 336-340, 2005. pdf
88. Kanchan A. Joshi, Joseph Wang, Robert Haddon, Wilfred Chen and Ashok Mulchandani., A disposable biosensor for organophosphorus nerve agents based on carbon nanotubes modified thick film strip electrode, Electroanalysis, 17, 54-58, 2005. pdf
87. Mark Shimazu, Wilfred Chen, Ashok Mulchandani. Biological detoxification of organophosphate pesticides. In Pesticide Decontamination and Detoxification, Gan, J.J., Zhu, P.C., Aust, S.D. and Lemley, A.T. (eds.). American Chemical Society Symposium Series, Vol. 863, 25-36, 2004.
86. Mark Shimazu, Wilfred Chen, Ashok Mulchandani. Biodegradation of organophosphate nerve agents. Phosphorus in Environmental Technologies: Principles and Applications. Eugenia Valsami-Jones (Ed.). IWA Publishing, London, UK. pp. 629-642, 2004.
85. Jianliang Zhang, Wensheng Lan, Chuanling Qiao, Hong Jiang, Ashok Mulchandani, and Wilfred Chen, Bioremediation of organophosphorus pesticides by surface-expressed carboxylesterase from mosquito on Escherichia coli, Biotechnol. Prog., 20, 1567-1571, 2004. pdf
84. Kumaran Ramanathan, Mangesh Bangar, Minhee Yun, Wilfred Chen, Ashok Mulchandani, and Nosang V. Myung, Individually addressable conducting-polymer nanowires array, Nano Letters, 4, 1237-1239, 2004. pdf
83. Jan Kostal, Rosanna Yang, Cindy H. Wu, Ashok Mulchandani, and Wilfred Chen, Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR, Appl. Environ. Microbiol., 70, 4582-4587, 2004. pdf
82. Kanchan A. Joshi, Prem C. Pandey, Wilfred Chen, and Ashok Mulchandani, Ormosil encapsulated pyrroloquinoline quinone-modified electrochemical sensor for thiols, Electroanalysis, 16, 1938-1943, 2004. pdf
81. Khaled H. Abd El Galil, M. A. El Sokkary, S. M. Kheira, Andre M. Salazar, Marylynn V. Yates, Wilfred Chen and Ashok Mulchandani, Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of Hepatitis A virus from environmental samples, Appl. Environ. Microbiol., 70, 4371-4374, 2004. pdf
80. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang, Ashok Mulchandani, Arthrobacter sp. JS443-Based Whole Cell Amperometric Biosensor for p-Nitrophenol, Electroanalysis, 16, 2030-2034, 2004. pdf
79. Giridhar Prabhukumar, Mark Matsumoto, Ashok Mulchandani, and Wilfred Chen, Cadmium Removal from Contaminated Soil by Tunable Biopolymers, Environ. Sci. Tech., 38, 3148-3152, 2004. pdf
78. Catherine Mee-Hie Cho, Ashok Mulchandani and Wilfred Chen, Altering the Substrate Specificity of Organophosphorus Hydrolase for Enhanced Hydrolysis of Chlorpyrifos, Appl. Environ. Microbiol., 70, 4681-4685, 2004. pdf
77. Joseph Wang, Gang Chen, Alexander Muck Jr., Madhu Prakash Chatrathi, Ashok Mulchandani, Wilfred Chen, Microchip enzymatic assay of organophosphate nerve agents, Analytica Chimica Acta, 505, 183–187, 2004. pdf
76. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang and Ashok Mulchandani, Whole cell-enzyme hybrid amperometric biosensor for organophosphorous nerve agents, Biotechnol. Bioeng., 85, 706-713, 2004. pdf
75. M. Thust, A., Mulchandani, J. Wang, M. Arzdorf, P. Mulchandani, W. Chen, and M.J. Schoning, Field-effect enzyme sensor for the detection of pesticides, TECHNISCHES MESSEN, 70, 561-564, 2003.
74. Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Affinity purification of plasmid DNA by temperature-triggered precipitation, Biotechnol. Bioeng., 85, 293-297, 2004. pdf
73. Jae-Young Kim, Ashok Mulchandani, and Wilfred Chen, An immunoassay for atrazine using tunable immunosorbent, Anal. Biochem., 322, 251-256, 2003. pdf
72. Michael. J. Schoning, Michael Arzdorf, Priti Mulchandani, Wilfred Chen, and Ashok Mulchandani, Towards a capacitive enzyme sensor for direct determination of organophosphorus pesticides: Fundamental studies and aspects of development, Sensor, 3, 119-127, 2003. pdf
71. Zhaohui Xu, Ashok Mulchandani, and Wilfred Chen, Detection of Benzene, Toluene, Ethyl Benzene, and Xylenes (BTEX) Using Toluene Dioxygenase-Peroxidase Coupling Reactions, Biotechnol. Prog., 19, 1812-1815, 2003. pdf
70. Jan Kostal, Ashok Mulchandani, Katie E. Gropp, and Wilfred Chen, A temperature responsive biopolymer for mercury removal, Environ. Sci. Tech., 37, 4457-4462, 2003. pdf
69. Sergi Morais, Sean O’Malley, Wilfred Chen, and Ashok Mulchandani, A tubulin-based fluorescence polarization bioassay for paclitaxel. Anal. Biochem., 321, 44-49, 2003. pdf
68. Mark Shimazu, Anne Nguyen, Ashok Mulchandani, and Wilfred Chen, Cell surface display of OPH in Pseudomonas putida using an ice-nucleation protein anchor, Biotechnol. Prog., 19, 1612-1614, 2003. pdf
67. Sergi Morais, Prem C. Pandey, Wilfred Chen and Ashok Mulchandani, A novel bioassay for screening and quantification of taxanes. Chem. Comm., 10, 1188-1189, 2003. pdf
66.
Micheal J. Schoning, Micheal Arzdorf, Priti
Mulchandani Wilfred Chen, and Ashok Mulchandani,
A capacitive field-effect sensor for the direct
determination of organophosphorus
pesticides, Sensors and Actuators B,
91, 92-97,
2003.
65.
Ibolya Bontidean, Josefin
Ahlqvist, Ashok Mulchandani, Wilfred Chen, Weon Bae, Rajesh K. Mehar,
Alessia Mortari, Elisabeth Csoregi, Novel synthetic phytochelatin-based
capacitive biosensor for heavy metal ion detection, Biosensor &
Bioelectronics, 18, 547-553, 2003.
pdf
61. Yu Lei, Priti Mulchandani, Wilfred Chen, Joseph Wang and Ashok Mulchandani, A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp., Electroanalysis, 15, 1160-1164, 2003. pdf
59. Joseph Wang, Robin Krause, Kirstin Block, Mustafa Musameh, Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Michael J. Schöning, Dual amperometric-potentiometric biosensor detection system for monitoring organophosphorus neurotoxins, Analytica Chimica Acta, 469, 197-203, 2002. pdf
58. Wilfred Chen and George Georgiou, Cell-Surface Display of Heterologous Proteins: From High Throughput Screening to Environmental Applications, Biotechnol. Bioeng., 79, 469-503, 2002. (Invited Contribution) pdf
57. Priti Mulchandani, Yu Lei, Wilfred Chen, Joseph Wang and Ashok Mulchandani, A microbial biosensor for p-nitrophenol using Moraxella sp., Analytica Chimica Acta, 470, 79-86, 2002. pdf
56. Zhaohui Xu, Weon Bae, Ashok Mulchandani, Rajesh K. Mehra, and Wilfred Chen, Heavy metal removal by novel CBD-EC20 sorbents immobilized on cellulose, Biomacromolecules, 3, 462-465, 2002. pdf
55. Catherine M-H. Cho, Ashok Mulchandani, and Wilfred Chen, Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents, Appl. Environ. Microbiol., 68, 2026-2030, 2002. pdf
54. Aijun A. Wang, Ashok Mulchandani, and Wilfred Chen, Specific adhesion to cellulose and hydrolysis of organophosphate nerve agents by a genetically engineered E. coli with surface-expressed cellulose-binding domain and organophosphorus hydrolase, Appl. Environ. Microbiol., 68, 1684-1689, 2002. pdf
53. Weon Bae, Rajesh Mehra, Ashok Mulchandani, and Wilfred Chen, Genetic engineering of Escherichia coli for enhanced bioaccumulation of mercury, Appl. Environ. Microbiol., 67, 5335-5338, 2001. pdf
52. Sung Hyo Chough, Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang and Kim R. Rogers, Organophosphorus hydrolase-based electrode for determination of organophosphate pesticides, Electroanalysis, 14, 273-276, 2002. pdf
51. Weon Bae, Ashok Mulchandani, and Wilfred Chen, Cell surface display of synthetic phytochelatins using ice nucleation protein for enhanced heavy-metal bioaccumulation, J. Inorg. Biochem., 88, 223-227, 2002. (Invited Contribution) pdf
50. Mark Shimazu, Ashok Mulchandani, and Wilfred Chen, Simultaneous Degradation of Organophosphorus Pesticides and p-nitrophenol by a Genetically Engineered Moraxella sp. with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Bioeng., 76, 318-324, 2001. pdf
49. Priti Mulchandani, Wilfred Chen, Ashok Mulchandani, Flow Injection Amperometric Enzyme Biosensor for Direct Determination of Organophosphate Nerve Agents, Environ. Sci. Tech., 35, 2562-2565, 2001. pdf
48. Joseph Wang, Madhu P. Chatrathi, Ashok Mulchandani, and Wilfred Chen, Capillary Electrophoresis Microchips for Rapid Separation and Detection of Organophosphate Nerve Agents, Anal. Chem., 73, 1804-1808, 2001. pdf
47. Ashok Mulchandani, Wilfred Chen, Priti Mulchandani, Joseph Wang, and Kim R. Rogers, Biosesnors for Direct Determination of Organophosphate Pesticides, Biosens. Bioelectron., 16, 225-230, 2001. (Invited Contribution) pdf
46. Priti Mulchandani, Wilfred Chen, Ashok Mulchandani, Joseph Wang and Liang Chen, Amperometric Microbial Biosensor for Direct Determination of Organophosphate Nerve Agents using Recombinant Moraxella sp. with Surface Expressed Organophosphorus Hydrolase, Biosens. Bioelectron., 16, 433-437, 2001. (Invited Contribution) pdf
45. Jan Kostal, Ashok Mulchandani, and Wilfred Chen, Tunable Biopolymers for Heavy Metal Removal, Macromolecules, 34, 2257-2261, 2001. pdf
44. Aijun Wang, Ashok Mulchandani, and Wilfred Chen, Whole Cell Immobilization using Surface-expressed Cellulose-Binding Domain, Biotechnol. Prog., 17, 407-411, 2001. pdf
43. Richard Richins and Wilfred Chen, Effects of FIS Overexpression on Cell Growth, rRNA Synthesis and Ribosome Content in Escherichia coli, Biotechnol. Prog., 17, 252-257, 2001. pdf
42. Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang, Liang Chen. Field-deployable enzyme electrodes for direct monitoring of organophosphate nerve agents. In: Enzymes in Heteroatom Chemistry: Green solutions for chemical problems. (Zwanenburg, B. et al., eds.), Kluwer Academic Publishers. The Netherlands, pp.287-296, 2000.
41. Wilfred Chen, Richard D. Richins, Priti Mulchandani, Irina Kaneva, and Ashok Mulchandani. Biodegradation of Organophosphorus Nerve Agents By Surface Expressed Organophosphorus Hydrolase. In: Enzymes in heteroatom chemistry: Green solutions for chemical reactions. (Zwanenburg, B. et al., eds.), Kluwer Academic Publishers. The Netherlands, pp. 211-222, 2000.
40. Ayman Mansee, Wilfred Chen, and Ashok Mulchandani, Biodetoxification of coumaphos insecticide using immobilized Escherichia coli expressing organophosphorus hydrolase enzyme on cell surface, Biotechnol. Bioprocess Eng., 5, 436-440, 2000. pdf
39. Mark Shimazu, Ashok Mulchandani, and Wilfred Chen, Cell Surface Display of Organophosphorus Hydrolase Using Ice Nucleation Protein, Biotechnol. Prog., 17, 76-80, 2001. pdf
38. Nathalie Fortin, Ashok Mulchandani, and Wilfred Chen, Use of real-time polymerase chain reaction and molecular beacon for the detection of Escherichia coli O157:H7, Anal. Biochem., 289, 281-288, 2001. pdf
37. Wilfred Chen, Ashok Mulchandani, and Joseph DeFrank, Biodegradation of Chemical Weapons, in Encyclopedia of Environmental Microbiology, John Wiley & Sons (invited contribution), 844-852, 2001.
36. Weon Bae, Wilfred Chen, Ashok Mulchandani, and Rajesh Mehra, Enhanced Bioaccumulation of Heavy Metal by Bacterial Cells Displaying Synthetic Phytochelatins, Biotechnol. Bioeng., 70, 518-524, 2000. pdf
35. Wilfred Chen, Grisselle Martinez and Ashok Mulchandani, Molecular Beacons: A New Approach for Detecting Salmonella Species, Sadik, O.A. and Mulchandani, A. (eds.), ACS Symposium Series No. 762, ACS, Washington, DC, pp.292-298, 2000.
34. Richard D. Richins, Ashok Mulchandani, and Wilfred Chen, Expression, Immobilization, and Enzymatic Characterization of Cellulose-Binding Domain-Organophosphorus Hydrolase Fusion Proteins, Biotechnol. Bioeng., 69, 591-596, 2000. pdf
33. Wilfred Chen, Grisselle Martinez, and Ashok Mulchandani, Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella, Anal. Biochem., 280, 166-172, 2000. pdf
32. Wilfred Chen, Fredi Bruhlman, Kelvin H. Lee and Marc A. Deshusses, Whole Cell Catalysis, in Encyclopedia of Catalysis, John Wiley & Sons (invited contribution), Vol. 6, 658-680, 2003.
31. Fredi Bruhlmann and Wilfred Chen, Transformation of Polychlorinated Biphenyl by a Novel BphA variant through the meta-cleavage pathway, FEMS Microbiol. Lett., 179, 203-208, 1999. pdf
30. Joseph Wang, Liang Chen, Ashok Mulchandani, Priti Mulchandani, and Wilfred Chen, Remote Biosensor for in-situ Monitoring of Organophosphate Nerve Agents. Electroanalysis 11, 866-869, 1999. pdf
29. Wilfred Chen and Ashok Mulchandani, Detoxification of pesticides, Biofutur, 187, 41-43, 1999.
28. Ashok Mulchandani, Priti Mulchandani, Wilfred Chen, Joseph Wang, and Liang Chen, Amperometric Thick-Film Strip Electrodes for Monitoring Organophosphate Nerve Agents Based on Immobilized Organophosphorus Hydrolase, Anal. Chem., 71, 2246-2249, 1999. pdf
27. Kim R. Rogers, Y. Wang, A. Mulchandani, P. Mulchandani, and Wilfred Chen, Organophosphorus Hydrolase-Based Assay for Organophosphate Pesticides, Biotechnol. Prog., 15, 517-521, 1999. pdf
26. Wilfred Chen, Fredi Bruhlmann, Richard Richins, and Ashok Mulchandani, Engineering of Improved Enzymes and Microbes for Bioremediation, Curr. Opin. Biotechnol., 10, 137-141, 1999. pdf
25. Fredi Bruhlmann and Wilfred Chen, Tuning Biphenyl Dioxygenases for Extended Substrate Specificity, Biotechnol. Bioeng., 63, 544-551, 1999. pdf
24. Ashok Mulchandani, Shengtian Pan and Wilfred Chen, Fiber-Optic Enzyme Biosensor for Direct Determination of Organophosphate Nerve Agents, Biotechnol. Prog., 15, 130-134, 1999. pdf
23. Leila H. Choe, Wilfred Chen and Kelvin H. Lee, Proteome Analysis of Fis Overproduction in Escherichia coli, Electrophoresis, 20, 798-805, 1999. pdf
22. Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Detoxification of Organophosphate Nerve Agents by Immobilized Escherichia coli with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Bioeng., 63, 216-223, 1999. pdf
21. Ashok Mulchandani, Priti Mulchandani and Wilfred Chen, Enzyme Biosensor for Determination of Organophosphates, Field Analytical Chemistry and Technology, 2, 363-369, 1998. pdf
20. Irina Kaneva, Priti Mulchandani, Richard Richins, Ashok Mulchandani, and Wilfred Chen. 1998. Biodetoxification and Monitoring of Organophosphorus Nerve Agents by surface-Expressed Organophosphorus Hydrolase. In Proceedings of the 1997 ERDEC Scientific Conference on Chemical & Biological Defense Research, 18-21 November 1997, ERDEC-SP-063, 251-255.
19. Priti Mulchandani, Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Biosensor for Direct Determination of Organophosphate Nerve Agents. 1. Potentiometric Enzyme Electrode, Biosens. Bioelectron., 14, 77-85, 1999. pdf
18. Ashok Mulchandani, Irina Kaneva and Wilfred Chen, Biosensor for Direct Determination of Organophsophate Nerve Agents Using Recombinant Escherichia coli with Surface-Expressed Organophosphorus Hydrolase. 2. Fiber-Optic Microbial Biosensor, Anal. Chem. 70, 5042-5046, 1998. pdf
17. Ashok Mulchandani, Priti Mulchandani, Irina Kaneva, and Wilfred Chen, Biosensor for Direct Determination of Organophosphate Nerve Agents Using Recombinant Escherichia coli with Surface-Expressed Organophosphorus Hydrolase. 1. Potentiometric Electrode, Anal. Chem., 70, 4140-4145, 1998. pdf
16. Ashok Mulchandani, Samir Chauhan, Irina Kaneva, Priti Mulchandani, and Wilfred Chen, A Potentiometric Microbial Biosensor for Direct Determination of Organophosphate Nerve Agents, Electroanalysis, 10, 733-737, 1998. pdf
15. Irina Kaneva, Ashok Mulchandani, and Wilfred Chen, Factors Influencing Parathion Degradation by Recombinant E. coli with Surface-Expressed Organophosphorus Hydrolase, Biotechnol. Prog., 14, 275-278, 1998. pdf
14. Wilfred Chen and Ashok Mulchandani, The Use of Live Biocatalysts for Pesticide Detoxification, Trends in Biotechnology, 16, 71-76, 1998. pdf
13. Richard Richins, Irina Kaneva, Ashok Mulchandani, and Wilfred Chen, Biodegradation of Organophosphorus Pesticides by Surface-Expressed Organophosphorus Hydrolase, Nature Biotechnology, 15, 984-987, 1997. pdf
12. Richard Richins, Tin Htay, Pauli Kallio, and Wilfred Chen, Elevated Fis Expression Enhances Recombinant Protein Synthesis in Escherichia coli, Biotechnol. Bioeng., 56, 138-144, 1997. pdf
11. Neilay Dedhia, Wilfred Chen and James E. Bailey, Design of Expression Systems for Metabolic Engineering: Coordinated Synthesis and Degradation of Glycogen, Biotechnol. Bioeng., 55, 419-426, 1997. pdf
10. Marc A. Deshusses, Wilfred Chen, Ashok Mulchandani, and Irving J. Dunn., Innovative Bioreactors, Curr. Opin. Biotechnol., 8, 165-168, 1997.
9. Neilay N.Dedhia, Richard Richins, Archie Mesina, and Wilfred Chen, Improvement in Recombinant Protein Production in ppGpp-Deficient Escherichia coli, Biotechnol. Bioeng., 53, 379-386, 1997. pdf
8. Wilfred Chen, Pauli Kallio and James Bailey, Process Characterization of a Novel Cross-Regulation System for Cloned Protein Production in Escherichia coli, Biotechnol. Prog., 11, 397-402, 1995. pdf
7. Wilfred Chen, Pauli Kallio, and James E. Bailey, Cross-Regulation of Gene Expression in Recombinant Cells , US Patent Number 5,416,800, 1995.
6. Wilfred Chen, Dallas E. Hughes and James E. Bailey, Intracellular Expression of Vitreoscilla Hemoglobin Alters Aerobic Metabolism of Saccharomyces cerevisiae, Biotechnol. Prog., 10, 308-313, 1994. pdf
5. Wilfred Chen and James E. Bailey, Application of the Cross-Regulation System as a Metabolic Switch, Biotechnol. Bioeng., 43, 1190-1193, 1994. pdf
4. Wilfred Chen, Pauli Kallio, S.B. Lee,. and James E. Bailey, Invention of a Useful Transcriptional Regulation System Using Mechanistic Mathematic Modelling, p. 155-158, in Modern Trends in Biothermokinetics (S. Schuster, M. Rigoulet, R. Ouhabi, and J.P. Mazat, Eds), Plenum Press, New York, U.S.A., 1993.
3. Wilfred Chen, Pauli Kallio and James E. Bailey, Construction and Characterization of a Novel Cross-Regulation System for Regulating Cloned-Gene Expression in E. coli, Gene, 130, 15-22, 1993. pdf
2. Neilay Dedhia, Thomas Hottiger, Wilfred Chen, and James E. Bailey, Genetic Manipulation of Central Carbon Metabolism in Escherichia coli, p. 59-62 in Harnessing Biotechnology for the 21st Century (M. R. Ladisch and A. Bose, Eds), American Chemical Society, New York, 1992.
1. Wilfred Chen, James E. Bailey and Sun Bok Lee, Molecular Design of Expression Systems: Comparison of Different Repressor Control Configurations Using Molecular Mechanism Models, Biotechnol. Bioeng., 38, 679-687, 1991. pdf